fa تحقيقي Original Paper <div> <p><strong>ABSTRACT</strong></p> <p>&nbsp;<strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Background and Objective:</strong> Biofilms are community of bacteria that attach to inanimate surfaces or living tissues via production of extracellular polymers and exopolysaccharide matrix. Microbial biofilms on various surfaces of the hospital environment are considered as a reservoir of infection spread. The present study aimed to evaluate the disinfecting effect of benzalkonium chloride on some bacterial isolates causing nosocomial infections.</p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Methods:</strong> First, 13 isolates from four bacteria including <em>Pseudomonas aeruginosa</em>, <em>Staphylococcus aureus, Acinetobacter</em> and <em>Enterobacter </em>were obtained from Microbiology Laboratory of Al-Zahra Hospital in Isfahan, Iran. The samples were transferred to Microbiology Laboratory of Faculty of Veterinary Medicine of Shahrekord University for testing. Evaluation of biofilm formation and determination of minimum inhibitory concentration (MIC) of the disinfectant and effect of the disinfectant on planktonic growth and biofilm formation were performed.</p> <p><strong>&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Results:</strong> All bacterial isolates (52 cases) produced biofilm. Mean MIC of benzalkonium chloride for <em>P. aeruginosa, S. aureus, Enterobacter</em> and <em>Acinetobacter</em> was 0.14, 0.2, 0.18, 0.17 g/ml, respectively. Planktonic growth of all four bacteria was inhibited at concentrations of 2MIC, MIC and 1/2MIC. Biofilm was not produced in MIC and 2MIC concentrations, and biofilm formation capability increased by reducing the concentration of benzalkonium chloride.</p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Conclusion:</strong> The results show that the use of appropriate concentration of benzalkonium chloride can prevent the growth of different bacterial species, but sub-MIC dose of this disinfectant may stimulate biofilm formation.</p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Keywords:</strong> Biofilm, Benzalkonium Chloride, <em>Pseudomonas Aeruginosa, Staphylococcus Aureus, Enterobacter, Acinetobacter.</em></p> </div> Biofilm, Benzalkonium Chloride, Pseudomonas Aeruginosa, Staphylococcus Aureus, Enterobacter, Acinetobacter 14 20 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-430&slc_lang=fa&sid=1 Azizollah Ebrahimi kahrizsangi 100319475328460012453 100319475328460012453 No Department of Pathobiology, Faculty of Veterinary Medicine Saied Habibian Dehkordi 100319475328460012454 100319475328460012454 No Department of Basic Sciences, Faculty of Veterinary Medicine Ziba Shabanpur 100319475328460012455 100319475328460012455 No Department of Bacteriology, Faculty of Veterinary Medicine Reza Hakimi Alni r.hakimi91@basu.ac.ir 100319475328460012456 100319475328460012456 Yes Department of Bacteriology, Faculty of Paraveterinary Majid Hemati 100319475328460012457 100319475328460012457 No Department of Bacteriology, Faculty of Veterinary Medicine