en ميکروب شناسي Microbiology Research Article Research Article <div style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Times New Roman;"><b>Background:</b> Post-operative wound infection has been a problem since surgery was started as a treatment modality and is the third most common cause of nosocomial infections with a reported incidence rate of 14-16%. This study aimed to investigate the prevalence, isolate and identify aerobic pathogenic bacteria from surgical site infections (SSI) and to determine the antibiotic susceptibility testing (AST) pattern of pathogenic bacteria.<br> <b>Methods:</b> This study was conducted at the Department of Microbiology SKIMS-Medical College, Bemina Srinagar, over a period of six months from November 2021 to April 2022. In the study, 210 samples from patients with SSI were included. Isolation, identification, and AST of the isolates were performed by standard microbiological techniques.<br> <b>Results:</b> Out of 210 SSI samples,<span style="color:black"> 163 bacterial isolates were recovered and </span>infection rate was more in 21-30 years age group (24.2%). <span style="color:black">Gram-negative bacteria were isolated in 50.4% (82/163) cases and <i>E. coli</i> was the most common organism (59.75%, 49/82). Gram-negative bacteria were sensitive to imipenem and none were resistant to polymyxin-B and colistin. <i>E. coli </i>was mostly resistant to cefoperazone, <i>Acinetobacter</i> and <i>Klebsiella species</i> were resistant to ceftazidime, <i>Pseudomonas </i>and <i>Citrobacter </i>were resistant to ceftriaxone. Gram-positive bacteria constituted 49.6% (81/163) and </span>Methicillin-resistant <i>Staphylococcus aureus </i><span style="color:black">(MRSA) was a frequently isolated species (66.6%, 54/81). MRSA- and </span>methicillin-sensitive <i>Staphylococcus aureus<span style="color:black"> (</span></i><span style="color:black">MSSA<i>) </i>were mostly sensitive to amikacin, gentamycin, and tetracycline. Moreover, none of the Gram-positive isolates were resistant to linezolid, vancomycin, and teicoplanin. <i>Enterococcus spp</i> was mostly resistant to gentamycin.</span><b><span style="font-size:8.0pt"></span></b><br> <b>Conclusion</b>: This study developed an insight into post-operative wound infections and their incidence, organisms&rsquo; prevalence, and their antibiogram. <span style="color:black">Culture positivity in suspected cases of SSI was high (77.6%). MRSA<i> and E. coli </i>were frequently isolated from Gram-positive and Gram-negative</span> <span style="color:black">bacteria.</span></span></span><span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><span style="font-size:8.0pt"></span></span></span></div> Surgical Wound Infection, Methicillin-Resistant Staphylococcus Aureus, Methicillin-Sensitive Staphylococcus Aureus 8 11 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1350-1&slc_lang=en&sid=1 Syed Majid Ali majidbukhari99@gmail.com 100319475328460029207 100319475328460029207 No Clinical Microbiology, Lovely Professional University Punjab, Punjab, India Jalila Qayoom jalilamalik@gmail.com 100319475328460029208 100319475328460029208 No Department of Microbiology Sher-i-Kashmir Institute of Medical Sciences, Medical College Bemina, Srinagar, India Talat Masoodi talatmasoodi@yahoo.co.in 100319475328460029209 100319475328460029209 No Department of Microbiology Sher-i-Kashmir Institute of Medical Sciences, Medical College Bemina, Srinagar, India Azhar Shafi zhip08@gmail.com 100319475328460029210 100319475328460029210 No Department of Microbiology Sher-i-Kashmir Institute of Medical Sciences, Medical College Bemina, Srinagar, India ; Jaipur National University, Jaipur, India Arshi Syed microskimsmc@gmail.com 100319475328460029211 100319475328460029211 Yes Department of Microbiology Sher-i-Kashmir Institute of Medical Sciences, Medical College Bemina, Srinagar, India