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Showing 2 results for Cytotoxic
Investigating Cytotoxic Effects of Juniperus Excelsa Extract on Esophageal Cancer Cell Line KYSE-30 and Normal Fibroblast Cell Line HU02
Ameneh Elikaei , Hossein Vazini, Fatemeh Javani Jouni , Jaber Zafari,
Volume 13, Issue 5 (9-2019)
Abstract
ABSTRACT
             Background and objectives: Esophageal cancer is the eighth most common type of cancer in the world. Considering the adverse effects of anticancer drugs and the emergence of chemotherapy resistance, plant-derived extracts and their constituents could be a valuable source of novel anticancer drugs. In this study, we investigated cytotoxic effects of Juniperus excelsa leaf extract on esophageal cancer cell line KYSE-30 and healthy fibroblast cells (HU02 cells).
             Methods: KYSE-30 cells and HU02 cells were cultured in DMEM medium. The cells were treated with different concentrations (1, 10, 100, 500 μg/ml) of the J. excelsa leaf extract for 24 and 48 hours. The cytotoxic effects of the extract were assessed using the MTT assay. Data were analyzed using SPSS (version 19) and GraphPad Prism 5.
             Results: According to results of the MTT assay, the Juniperus excelsa’s leaf extract exerted significant cytotoxic effects on esophagus cancer cell line (KYSE-30) and healthy fibroblast cells (HU02) in a time- and dose-dependent manner (P<0.05).
             Conclusion: The J. excelsa leaf extract has cytotoxic effects against KYSE-30 esophageal cancer cells while causing lesser toxicity on healthy fibroblast cells. Our findings suggest that the potential anticancer effects of this extract should be further exploited in future studies.
             Keywords: Cytotoxic, MTT, Hu02, Kyse-30, Juniperus excelsa.

Effect of urtica dioica agglutinin on breast cancer cell growth
Esmail Samadian , Arash Golalipour , Mohammadali Vakili , Hasan Mohammed , Azam Rashidbaghan ,
Volume 20, Issue 3 (7-2026)
Abstract
Background: Breast cancer remains a leading cause of mortality among women globally, necessitating the exploration of novel therapies with minimal side effects. Urtica dioica agglutinin (UDA), a lectin derived from stinging nettle, exhibits antiproliferative properties in various cancers; however, its effects on breast cancer cells remain underexplored. This study evaluates the cytotoxic potential of UDA against MCF-7 breast cancer cells while assessing its impact on normal mammary (MCF-10A) and embryonic kidney (HEK-293) cells.
Methods: UDA was purified from Urtica dioica rhizomes by affinity chromatography and confirmed by SDS-PAGE (8.5 - 9.5 kDa) and agglutination assays. MCF-7, MCF-10A, and HEK-293 cells were treated with different concentrations of UDA (7.5 - 480 µg/mL) for 24 and 48 hours. Cytotoxicity was assessed using MTT assays to measure cell viability.
Results: UDA significantly inhibited MCF-7 proliferation in a dose- and time-dependent manner (P < 0.01 at 24 hours; P < 0.0001 at 48 hours). At 240 µg/mL (During 48 hours), viability dropped below 50%, while normal HEK-293 cells showed < 30% toxicity. MCF-10A proliferation remained unaffected, even at 480 µg/mL.
Conclusion: UDA selectively targets breast cancer cells (MCF-7) with minimal toxicity to normal cells, positioning it as a promising anticancer candidate. Further studies are needed to elucidate its mechanism of action and apoptosis-inducing potential.

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