fa مولکولار اپیدمیولوژی پاپیلوماویروس انسانی و انواع پرخطر آن(HPV-16, ( 18 در بافت سرطانی پستان به روشPCR در استان گلستان Abstract Background and objectives: Breast cancer is the most prevalent one in women. Some of the common causative factors are genetic background, nutritional and environmental factors. Viruses are believed as a risk factor in this cancer, too. Recent studies reported that Human Papillomaviruses can be one of the possible risk factors of breast cancer. This study focused on investigation of the papillomavirus genome in tissues of breast cancer in Golestan province, Iran. Material and Methods: This descriptive analytical study was done from 2005 until 2008. The Samples were obtained from women admitted to the hospitals in Gorgan and Gonbad cities. All breast biopsy or mastectomy tissues were confirmed by the pathologists for breast cancer. DNA was extracted and PCR done by using general primers (GP5 + / GP6 + and MY09/MY11) for detection of papillomavirus genomes. Results: The Subjects are 231 patients aged 47± 12/72, the youngest 20 and the oldest 84. They are from Gorgan (N=122)and Gonbad (N=109) The result Shows That The Subjects Suffer from infiltrating ductular Carcinoma(31.4%), infiltrating duct Carcinoma (60.1%)and intraductal Lobular Carcinoma (4.3%) and The rest from other kinds of Cancer. Papilloma Virus genome is not found in These Samples. Conclusion: Based on paradoxical results from different parts of the world, upon the presence or absence of papillomavirus genome in breast cancer samples, to show the role of this virus in the development of breast cancer more studies are needed. Key words: Breast Cancer, Papillomaviruses, Golestan Province Key words: Breast Cancer, Papillomaviruses, Golestan Province 0 0 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-29&slc_lang=fa&sid=1 A Moradi عبدالوهاب مرادی abmoradi@ yahoo.com 100319475328460013157 100319475328460013157 Yes Associate professor, Microbiology دانشگاه علوم پزشکی گلستان،دانشکده پزشکی ،گروه میکروبولوژی E Mobasheri الهام مبشری 100319475328460013158 100319475328460013158 No دانشگاه علوم پزشکی گلستان A Tabarraei علیجان تبرائی 100319475328460013159 100319475328460013159 No دانشگاه علوم پزشکی گلستان S Bakhshandeh Nosrat سپیده بخشنده نصرت 100319475328460013160 100319475328460013160 No مرکز تحقیقات عفونی ، دانشگاه علوم پزشکی گلستان V Kazemi nejad وحیده کاظمی نژاد 100319475328460013161 100319475328460013161 No -مرکز تحقیقات عفونی دانشگاه علوم پزشکی گلستان R Azarhosh رامین آذرهوش 100319475328460013162 100319475328460013162 No دانشگاه علوم پزشکی گلستان SH Alizadeh شهریار علیزاده 100319475328460013163 100319475328460013163 No M Bazori مسعود بازوری 100319475328460013164 100319475328460013164 No دانشگاه علوم پزشکی گلستان